Part:BBa_K2605007
CMV with 5' SalI restriction site + monomeric RFP optimized for bacteria
Cytomegalovirus (CMV) promoter with 5' SalI restriction site fused to mCherry red fluorescent protein optimized for expression in prokaryotes.
Usage and Biology
This part was made to test the function of an improved CMV promoter Part:BBa_K2605001.
Digest-confirmed CMV-RFP constructs and mCherry alone (Part:BBa_J06504, as a negative control) were transformed into chemically competent E. coli DH5-alpha cells. Successful transformants were plated and imaged after 48h, at which time RFP production could be seen in both CMV-RFP constructs (figure 1, figure 2), while the negative control remained colourless (figure 1) as RFP could not be produced without a CMV promoter.
Figure 1. Streak plates of E. coli DH5-alpha containing TOP: pSB1C3-BBa_J06504 (RFP) fused to BBa_K747096 (CMV), RIGHT: pSB1C3-BBa_J06504 (RFP) fused to BBa_K2605001 (CMV) LEFT: negative control, pSB1C3-BBa_J06504 (RFP).
Figure 2. Streak plates of E. coli DH5-alpha containing TOP: pSB1C3-BBa_J06504 (RFP) fused to BBa_K747096 (CMV), BOTTOM: pSB1C3-BBa_J06504 (RFP) fused to BBa_K2605001 (CMV)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 565
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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